The protein SHC1 also acts as a scaffold protein which is used in cell surface receptors. Such reference data will be useful control data for all hydrogen exchange mass spectrometry experiments involving nanodiscs in which transmembrane or lipid-associated proteins are the primary molecule(s) of interest. These proteins differ in activity and subcellular locations, p66 is the longest and while the p52 and p46 link activated receptor tyrosine kinase to the RAS pathway. This work provides a catalog of the expected scaffold protein peptic peptides in a nanodisc-hydrogen exchange mass spectrometry experiment and their deuterium uptake signatures, data that can be used as a benchmark to verify correct assembly and nanodisc structure. The rapid deuteration of the scaffold protein and the presence of correlated unfolding events both indicate that nanodiscs are dynamic rather than rigid bodies in solution. Scaffold protein definition: a temporary metal or wooden framework that is used to support workmen and materials. An RNA-Binding Protein, hnRNP A1, and a Scaffold Protein, Septin 6, Facilitate Hepatitis C Virus Replication. Scaffold proteins help relay the message between the cell. Several regions of the scaffold protein, in both the lipid-free and lipid-associated states, displayed EX1 unfolding kinetics. Protein scaffolds are members of the signaling cascade downstream of cell surface receptors. They do this by serving as a docking site for multiple protein partners in the cascade so they can be near each other. Scaffold proteins help relay the message between the cell membrane and nucleus faster. This protection was short-lived and the scaffold protein was highly deuterated within 2 h. Protein scaffolds are members of the signaling cascade downstream of cell surface receptors. Modules are compact folded portions of proteins with 30200 amino acids that recognize short continuous peptide sequences in their binding partners. On nanodisc self-assembly, the entire scaffold protein gained significant protection from exchange, consistent with a large, protein-wide, structural rearrangement. Scaffold proteins are composed of multiple protein modules. which are built on the protein A scaffold, can be effectively used as. Although scaffolds are not strictly defined in function, they are. Hydrogen exchange mass spectrometry was used to probe the structure and dynamics of the scaffold protein in the presence and absence of lipid. to note that these small synthetic binding proteins are no more foreign than the. In biology, scaffold proteins are crucial regulators of many key signalling pathways. One practical concern is the increasing scaffold protein size to. Nanodiscs consist of a discoidal phospholipid bilayer encircled by two copies of an amphipathic alpha helical membrane scaffold protein, which is modeled from apolipoprotein A-1. of scaffolding complex mobility, suggested from lowered activity with the addition of. Phospholipid bilayer nanodiscs are model membrane systems that provide an environment where membrane proteins are highly stable and monodisperse without the use of detergents or liposomes.